Laboratory Instrumentation

The Natural Sciences Laboratories and Field Facilities support practical learning experiences that fully complement the science courses and academic programs offered by the University.

 

Instrumentation

The Biology and Chemistry programs are housed mainly in the Unified Science Center where introductory and advanced laboratory courses in these disciplines are offered. Advanced instrumentation and research rooms are located within the Unified Science Center. Undergraduate teaching and research utilize the following instrumentation and equipment:

 

Agilent 1260 High Performance Liquid Chromatograph (HPLC) with diode array detector 

A pressure range of up to 600 bar with a flow rate up to 
5 mL/min allows the use of almost any column – conventional, sub-2 µm-particle or superficially porous columns. A UV, fluorescence or ELSD detector provides the data rate required for high resolution separations or fast analyses. 

Image of Agilent 1260 High Performance LIquid Chromatograph HPLC with diode array detector

Molecular Devices Gene Pix 4100A  

Genomics

  • SNP genotyping arrays
  • Arrayed comparative genomic hybridization (aCGH) arrays

Transcriptomics

  • Gene expression arrays
  • RNA interference (RNAi) arrays
  • MicroRNA (miRNA) arrays

Proteomics

  • Protein arrays
  • Peptide arrays
  • Antibody arrays
  • ELISA arrays
  • Reverse Phase Arrays

Epigenomics

  • DNA methylation arrays
  • Chromatin immunoprecipitation (ChIP) arrays

Novel Applications

  • Tissue arrays
  • Quantum Dots
  • Carbohydrate Arrays
  • Chemical Compound Arrays

Image of Molecular dvices Gene Pix 4100A and Spectramax M5 microplate reader spectrophotometer

Spectramax M5 microplate reader Spectrophotometer

The optical systems use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to
that of dedicated single-mode readers.

Source: Molecular Devices: SpectraMax M Series Multi-Mode Microplate Readers Manual and Molecular Devices GenePix 4100A Microarray Scanner website

Perkin Elmer Tri Carb 3110TR Liquid Scintillation Analyzer

The Tri-Carb® 3110TR is a computer-controlled benchtop liquid scintillation analyzer for detecting small amounts of alpha, beta and gamma radioactivity.

 

Source: PerkinElmer Tri-Carb 3110TR Low Activity Liquid Scintillation Analyzer Spec Sheet

Image of the Tri Carb 3110TR liquid Scintillation Analyzer

 

Applied Biosystems Hitachi 3500 Genetic Analyzer

The Applied Biosystems 3500/3500xL Genetic Analyzers are fluorescence based DNA analysis instrument using capillary electrophoresis technology with 8- or 24-capillaries.

Source: Applied Biosystems 3500/3500XL Genetic Analyzer User Guide
Image of the Hitachi 3500 Applied Biosystems Genetic Analyzer

 

Beckman CEQ8000XL Automated DNA Sequencer

This system is fully automated and capable of determining the base sequence and fragment length of DNA samples that have been prepared with dye-labeled reagents. Four-color, dye-labeled terminator chemistry kits are used to process samples for base sequence analysis. Generation of samples for fragment length analysis is performed using dye-labeled primers.

Source: CEQTM 8000 Genetic Analysis System User's Guide

Image of Beckman CEQ8000XL Automated DNA Sequencer

 

The Biomek® 3000 Laboratory Automation Workstation

The Biomek® 3000 Laboratory Automation Workstation is a multi-axis liquid handling instrument designed for benchtop use and to fit in a laminar flow or fume hood for sterile or hazardous operations. The open architecture design, along with the extensible operating software, provides a foundation for integrating current and future specific-use components.

The Biomek 3000 workstation is a single head instrument with a series of interchangeable tools. Different tools provide options for performing a variety of functions, including liquid transfer and plate washing operations and moving labware around the deck. The modular design of the Biomek 3000 workstation allows expansion of the instrument capabilities to include additional operations such as filtration, plate shaking, photometric microplate measurement, and high-capacity operation.

Image of the Beckman Biomek 3000 Laboratory Automation Workstation

Image of the Beckman Biomek 3000 Laboratory Automation Workstation

A variety of labware and hardware adapt the deck of the Biomek 3000 workstation to accomplish multiple tasks, ranging from performing simple labware positioning and liquid transfers to completing complex assays that typically require additional devices in the laboratory.

Source: Beckman Coutler Biomek® 3000 Laboratory Automation Workstation User's Manual

 

Coy Anaerobic Chamber

Used for anerobic microbiology research, these units are also critical to many other research areas such as protein purification, clinical microbiology, biochemistry, cell culture, human microbiome studies, biofuels and more. 

Source: Coylab.com/products/anareobic-chambers
 Image of student in front of Coy Anaerobic Chamber

 

Leica Microsystems Microscope

Precise 5W LED illumination ideal for cell and tissue culture, micromanipulation, and live cell examinations.

Source: Leica Microsystems
Image of student looking through the Leica Microsystems Microscope 

Molecular Devices Gene Pix 4100A  

Genomics

Transcriptomics

Proteomics

Epigenomics

Novel Applications

Source: Moleculardevices.com

Spectramax M5 microplate reader Spectrophotometer

The optical systems use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to
that of dedicated single-mode readers.

Source: Spectramax M Series Multi-Mode Microplate Readers User Manual

Image of Molecular dvices Gene Pix 4100A and Spectramax M5 microplate reader spectrophotometer

Seal Analytical AA3 Dissolved Nutrient Analyzer

Based on air Segmented Flow Analysis techniques (also known as Continuous Flow Analysis), the SEAL AutoAnalyzer 3 fully automates repetitive and complex sample analysis steps, from start-up to shutdown. The SEAL AA3 HR chemistry analyzer can also perform solvent extraction, distillation, gas diffusion, on-line filtration and in-line UV digestion in a continuously flowing stream. SEAL AA3 HR chemistry analyzers are fully supported with a library of over 700 documented applications including USEPA, ISO and ASTM standard methods.

Source: SealAnalytical.com

Image of Seal Analytical AA3 dissolved nutrient analyer

Image of Seal Analytical AA3 dissolved nutrient analyzer

 

XROMM Analyzer

X-Ray Reconstruction of Moving Morphology (XROMM) is a 3D imaging technology for visualizing rapid skeletal movement in vivo

XROMM combines 3D models of bone morphology with movement data from biplanar x-ray video to create highly accurate (±0.1 mm) re-animations of the 3D bones moving in 3D space. 

Rapid bone motion, such as during bird flight, frog jumping, and human running, can be visualized and quantified with XROMM.

Source: Xromm.org

Image of the XROMM Analyzer

Image of the XROMM Analyzer mobile c-arm fluroscope

 

Agilent 1260 High Performance Liquid Chromatograph (HPLC) with diode array detector 

A pressure range of up to 600 bar with a flow rate up to 
5 mL/min allows the use of almost any column – conventional, sub-2 µm-particle or superficially porous columns. A UV, fluorescence or ELSD detector provides the data rate required for high resolution separations or fast analyses. 

Source: labwrench.com

Image of Agilent 1260 High Performance LIquid Chromatograph HPLC with diode array detector

Agilent 7890B Gas Chromatographs (GC) with autosamplers and a variety of detectors: ECD, TCD, FID, MSD

Gas chromatography, coupled with static
headspace sampling, is an easy-to-use,
high-throughput tool for determining
residual solvent impurities in pharmaceutical
products. Sample preparation is simple, and
the method is easily validated. In addition,
headspace sampling allows you to avoid
matrix injections that can cause column
degradation and coelution.

Source: Agilent 7890B Gas Chromatograph brochure

 Image of Agilent 7890B Gas Chromatographs (GC) with autosmaplers and a variety of detectors: ECD, TCD, FID, MSD

Image of Agilent 7890B Gas Chromatographs (GC) with autosamplers and a variety of detectors: ECD, TCD, FID, MSD

 

 

Agilent Cary Eclipse Fluorescence Spectorphotometer

The fibre optic capabilities extend the utility of the system for simplified measurement of solid samples outside the sample compartment or for the direct measurement of cold, hot, or potentially dangerous samples, down to very low volumes.

Food
  • Additives and supplements. Quality
    Assurance testing and monitoring
  • Authenticity and food origin
  • Food packaging and processing
    including hydrocarbon contamination
    through processing
Environmental
  • Preliminary Identification of hydrocarbon
    origin in oil spills using synchronous
    scanning
  • Waste water tracing into environmental
    flows by using fluorescent markers
  • Investigation of the source of organic
    matter pollution in river and sea water

Image of Agilent Cary Eclipse Flurescence Spectorphotometer

Chemical and Materials Applications
  • Determination of fluorescent properties
    of cleaning products
  • Investigation of the fluorescent
    properties of optical components
  • Analysis of surface contamination by
    fluorescent organic compounds in
    manufacturing processes
  • Polarised (anisotropy) measurements
    for understanding the molecular
    environment during polymer research
Source: Agilent Cary Eclipse Fluorescence Spectrophotometer spec sheet

Agilent 240 FS AA Flame Atomic Absorption Spectrophotometer Hollow Cathode Lamps

Atomic absorption spectrophotometry analyzes the concentration of elements in a liquid sample based on energy absorbed from certain wavelengths of light (usually 190 to 900 nm). Atomic absorption spectrophotometers typically include a flame burner to atomize the sample (most commonly a hollow cathode lamp), a monochromator, and a photon detector. Equipped with a turret or fixed lamp socket that can hold multiple lamps (up to eight) to reduce downtime between samples or allow for sequential analysis.

Typical sensitivity for an atomic absorption spectrometer using a flame burner is in the parts per million range. For trace analysis, a graphite furnace can be used in place of a flame burner to increase the sensitivity by several orders of magnitude (in the parts per billion range). Atomic absorption spectrophotometers are used in many industries including environmental testing, metal analysis, semiconductor manufacturing, petroleum and chemical production, and in pharmaceuticals, for example.

Source: labcompare.com

Image of Agilent 240 FS AA Flame Atomic Absorption Spectrophotometer Hollow Cathode Lamps

Bruker Advance 400 MHz Nuclear Magnetic Resonance (NMR) Spectrometer

Analytical nuclear magnetic resonance (NMR) solutions and instruments for life science and material research applications.

Nuclear magnetic resonance spectroscopy is used to study the structure of molecules, the interaction of various molecules, the kinetics or dynamics of molecules and the composition of mixtures of biological or synthetic solutions or composites.

The size of the molecules analyzed can range from a small organic molecule or metabolite, to a mid-sized peptide or a natural product, all the way up to proteins of several tens of kDa in molecular weight.

NMR nuclear spectroscopy complements other structural and analytical techniques such as X-ray, crystallography and mass spectrometry. NMR’s advantage is the unique ability of a nuclear spectrometer to allow both the non-destructive and the quantitative study of molecules in solution and in solid state, as well as to enable the study of biological fluids.

Source: bruker.com

 

 IMage of Bruker Advance 400 MHz NMR Spectrometer

CEM MARS 6 Microwave system

Microwave is a technique used to dissolve solid sample matrices into an aqueous liquid. This is achieved by placing a sample in a concentrated acid matrix in a closed vessel and exposing it to microwave irradiation. Both the speed of thermal decomposition of the sample, and the solubility of metals are increased. Once these metals are in solution, they can be quantified through spectroscopic techniques. The MARS 6 acid digestion process routinely cuts the time of sample preparation by 50 – 75 % as compared to hot plates and hot block.

Source: CEM.com

Image of the CEM MARS 6 Microwave System

 

Dionex 5000 Ion Chromatographs (IC)

Ideally suited for trace level determinations with high matrix concentrations, using a standard or microbore column in the first dimension to separate analytes from matrix, followed by a capillary separation in the second dimension. This method provides high sensititivy, enabling conductivity detection limits to rival those of mass speectrometry.

Source: ThermoFisher.com 

 

Image of Dionex 500 Ion Chromatograph (IC)

 

 

TA Instruments Q10 Differential Scanning Calorimeter (DSC)

The most common DSC application is the precise measurement of transition temperature.
Whether a melting temperature of a polymer
or the polymorphic transition of a pharmaceutical,
DSC provides the information quickly and easily on a minimum amount of sample. Important temperature measurements include:

  • Melting Temperature
  • Glass Transition Temperature
  • Thermal Stability Temperature
  • Oxidation Onset Temperature
  • Cure Onset Temperature
  • Crystallization Temperature
  • Polymorphic Transition Temperature
  • Liquid Crystal Temperature
  • Protein Denaturation Temperature
  • Solid-Solid Transition Temperature
Source: tainstruments.com/brochure

Image of Beckman CEQ8000XL Automated DNA Sequencer

Teledyne Leeman Labs Hydra II Mercury Analyzer

It is a fully automated turnkey analyzer that measures mercury in solid and semi-solid sample matrices directly without any acid digestion (sample preparation). The system employs the technique of sample combustion (thermal decomposition), mercury concentration via gold amalgamation and detection by cold vapor atomic absorption.

Source: teledyneleemanlabs.com

Image of Teledyne Leeman Labs Hydra II Mercury Analyzer

 

Thermo Nicolet iS5 FTIR with ATR attachment

Infrared spectroscopy is the analytical technique for rapid identification of unknown materials (solids, liquids and gases) and product quality screening. 

Image of Thermo Nicolet iS5 FTIR with ATR attachment

Source: fishersci.com

Image of Thermo Nicolet iS5 FTIR with ATR attachment

 

Thermo Nicolet iS5 FTIR Spectrometer with ATR and multi-range beam splitter

The Nicolet iS50 analyzes complex mixtures and materials using its GC-IR or TGA-IR capabilities. The new OMNIC Mercury software automatically isolates and identifies compounds eluted from a GC column or evolved from a TGA. This functionality is especially useful in TGA, where several compounds can evolve at the same time. Such analyses provide insight into subtle formulation differences in plastics and rubbers, which can lead to not-so-subtle differences in product performance and quality.

Source: News-medical.net

IMage of Thermo Nicolet iS50 FTIR with ATR and multi-range beam splitter

 

Rigaku MiniFlex 600 X-ray Diffractometer (powder) with 6-sample changer

MiniFlex X-ray diffractometer (XRD) is a multipurpose analytical instrument that can determine: phase identification and quantification, percent (%) crystallinity, crystallite size and strain, lattice parameter refinement, Rietveld refinement, and molecular structure. It is widely used in research, especially in material science and chemistry, as well as in industry for research and quality control.

Source: Rigaku.com 

Image of Rigaku MiniFlex 600 X-ray Diffractometer (powder) with 6-sample changer

Rigaku XtaLAB mini X-ray Diffractometer (single crystal) (XRD)

The Rigaku XtaLAB, benchtop X-ray crystallography system, is a compact single crystal X-ray diffractometer designed to produce publication-quality 3D structures. Rapidly analyze new compounds as they are synthesized in the lab.

Source: Rigaku.com

IMage of Rigaku XtaLAB mini X-Ray Diffractometer (single crystal)

Coming Soon

 

 

Coming Soon

 

 

Coming Soon

 

 

NanoMagnetics Atomic Force Microscope (AFM)

Create images of atoms and map the surface structure materials. Offers Excellent features such as alignment-free design, closed loop flexure scanner, decoupled z scanner, 10 MP video microscope and flexible operating modes.

Source: Nanomagnetics Atomic Force Microscope Flyer

 Image of Stockton University physics instrumentation, atomic force microscope

More Coming Soon

 
 

Coming Soon

 

 

Facilities

The School of Natural Sciences and Mathematics (NAMS) requires an extensive on-campus and off-campus infrastructure to accomplish its academic mission in the different scientific disciplines.

During the academic year 2016-17 the NAMS Lab courses served 1,891 unique students in 2830 lab seats in the fall and 1,691 students in 2,525 seats. These numbers include students who were enrolled in Independent Study courses having a laboratory or field facility component during the same period of time. These numbers show a general increase overall from the 2012-13 academic year.  

Teaching spaces are in different locations of the main campus and off campus facilities. On the main campus, NAMS has two distinctive and separate administrative offices; the NAMS Academic Administration Office (in the USC1, Room 240) and the NAMS Science Laboratories Office (in the lower F-wing, F-001). The School operates and supports research and teaching laboratories serving Biology (F-wing, USC1, A&S), Chemistry (F-wing, USC1), Marine Science (F-wing, MFS) Physics (lower C-wing), Mathematics  (main campus, F-wing), Sustainability (lower F-wing and Sustainability Farm),  as well as Environmental Science and Geology in the Arts and Science (A&S, Arboretum, MFS).      Support facilities are:  the greenhouses, the vivarium, chemical prep laboratories, and several scientific instrument laboratories. A 20-station Geographic Information Systems (GIS) in the A&S building) as well as several specialized computer laboratories in F-wing and USC1 add to the service provided to accomplish the teaching and research missions of NAMS.

The Marine Science and Environmental Field Station is located off Route 9 in the town of Port Republic, 8 miles from the main campus, on the Nacote Creek. The Nacote Creek is a tributary to the pristine Mullica River-Great Bay estuary which, was designated by Congress as the Jacques Cousteau National Estuarine Research Reserve (JCNERR), one of twenty-two such designated sites in the country.  The 7.8-acre field station site is at both sides of Wilson Avenue just off Route 9.  Two acres of the property at the south side of the street features a 250 feet waterfront with a 100 by 50 foot bulk-headed lagoon and two buildings; a two story structure, Building 504 houses a wet laboratory and aquaria on the lower floor and staff offices on the second floor.  It is also the location of the University’s Coastal Research Center.

On the north side of Wilson Avenue there is a 2000 square feet log cabin, renovated in 1997, with help from a grant from the National Science Foundation, into a teaching laboratory, faculty research areas and a research greenhouse.  A full description is given later in this report.

The Vivarium is located in F-wing and supports a variety of research and display animals as well as the rehabilitation and incubation for the terrapin project.  This facility also cares for mice under an NIH funded research project.  Many different species of rodents, fish, amphibians, and reptiles are also housed in the area.  This area is secured to only staff working and trained in handling the animals and monitored and maintained throughout the day.  TES and Student workers assist with cleaning the area, feeding and caring for the animals, as well as recording documentation of the animal’s care.  One full-time staff and one part-time staff oversee the operation 7 days a week. 

The Greenhouses are located at three different areas on campus and used for very different types of horticulture.  F-wing greenhouse is the main potted plant area used to grow and care for a very large variety of plants used for display and teaching.  This area is maintained  by two staff members and a variety of student workers.  The A&S greenhouse is a small addition to the building with a much warmer climate and used for soil analysis within the GEOL and ENVL classes.  One part-time staff member oversees this greenhouse operation.  The Sustainability farm on campus has a large mobile greenhouse that allows a variety of plants to be grown inside part of their life and outside for the rest.  This is a great experimental area of research and the greenhouse extends the possible time for growing.  The sustainability farm and its green house is run by one full-time staff member working closely with faculty, TES, and Student workers.  These green houses are used extensively for research and classroom activities.  The USC2 will have a large green house on the 3rd floor with a more spacious layout and work area to accommodate more variety of plants and better temperature control between areas.  This new greenhouse will replace the space in F-wing and be more accessible for students in classes and outreach events.

The Observatory located off Pomona Road, on the west campus, houses a 0.4 m Meade computer controlled telescope.  The Observatory is used for the teaching of General Astronomy courses to an average of 60 students per semester.  The course is also offered in one of the summer sessions when in demand.

The Arboretum is a 3-acre area plot located off Vera King Farris Drive on the southern part of the campus.  It is surrounded by an electric fence enclosing over 80 specimens of trees and shrubs, and eight raised beds containing many display and medicinal plants.  The area has an underground pipe network to support a computer controlled irrigation system.  The Arboretum’s plantings are managed by Professor George Zimmerman who has obtained donations from local nurseries, and was also awarded a Distinguished Faculty Fellowship to erect five shade structures for his white cedar studies.

The Sustainability Farm was established in 2012 as a student-run project. The farm operated under the leadership of a student farm manager until 2016. The student farm manager was responsible for ordering seeds and materials, crop planning, organizing labor and academic involvement, and managing the farm budget. Farm work was accomplished by volunteers during the academic year and 1-3 interns during the summer months. Varying degrees of success were achieved under this system. Success was measured by student and campus engagement, as well as the general functionality of the Stockton Farm as a farm – growing crops, managing pests and weeds, harvesting produce, and providing opportunities for academic challenge and growth.

The campus Sustainability Coordinator, as well as a faculty member in the SUST program, took over farm management in May 2016. In the year following the change in leadership, farm productivity and campus visibility increased relative to years previous. Infrastructure expanded to include a 30x48’ high tunnel, increased water storage, increased solar power and storage, and a new fence increasing production area from one-third of an acre to 1.5 acres. A new structure for student involvement was introduced; students are now enrolled in a Farm Practicum class each semester, which ensures their commitment and formalizes the academic aspect of farm participation. Enrolled students are given the opportunity to go on a field trip each semester in order to observe other farm and food systems in our region.

The farm also boasts having a large mobile greenhouse structure that can be moved to create different environments throughout the seasons.  This allows for earlier/later growing seasons for certain crops, or longer seasons at a more consistent temperature.  This structure helps to create a better scientific experience for those learning on the farm.

 The Spring of 2018 will bring the opening of the Unified Science Center 2 (USC2) The state-of-the-art facilities will allow Stockton to accommodate more students and better prepare them for successful careers in science, technology, engineering and mathematics (STEM).  The academic quad expansion will include the $33.2 million USC2 and a $15.2 million Health Sciences Center, near the existing Unified Science Center and Campus Center. The new buildings will be supported by funding from the Building Our Future Bond Act, which was overwhelmingly approved by New Jersey voters in 2012. The bond act will provide $21.465 million in funding for the USC2 and the University will pay 25 percent or $7.155 million. The Health Sciences Center will receive $13.5 million in funding from the bond issue and the University will pay 25 percent or $4.5 million.

The main entrance of the academic quad will face Vera King Farris Drive and will provide a central location for students to study and meet between classes, as well as space for the University community to gather for campus events.  The 58,210 thousand square foot Unified Science Center 2 will be an expansion to the existing 64,000-square-foot Unified Science Center. The three-story building will house teaching and research labs for various disciplines in the sciences, a vivarium, a large green house, a multi-purpose room and faculty offices. The 37,720-square-foot Health Sciences Center will include space for the Sustainability program, classrooms, faculty offices and collaboration areas with tables and chairs.