Laboratory Instrumentation

The Natural Sciences Laboratories and Field Facilities support practical learning experiences that fully complement the science courses and academic programs offered by the University.

 

Instrumentation

The Biology and Chemistry programs are housed mainly in the Unified Science Center where introductory and advanced laboratory courses in these disciplines are offered. Advanced instrumentation and research rooms are located within the Unified Science Center. Undergraduate teaching and research utilize the following instrumentation and equipment:

 

Agilent 1260 High Performance Liquid Chromatograph (HPLC) with diode array detector 

A pressure range of up to 600 bar with a flow rate up to 
5 mL/min allows the use of almost any column – conventional, sub-2 µm-particle or superficially porous columns. A UV, fluorescence or ELSD detector provides the data rate required for high resolution separations or fast analyses. 

Image of Agilent 1260 High Performance LIquid Chromatograph HPLC with diode array detector

Molecular Devices Gene Pix 4100A  

Genomics

  • SNP genotyping arrays
  • Arrayed comparative genomic hybridization (aCGH) arrays

Transcriptomics

  • Gene expression arrays
  • RNA interference (RNAi) arrays
  • MicroRNA (miRNA) arrays

Proteomics

  • Protein arrays
  • Peptide arrays
  • Antibody arrays
  • ELISA arrays
  • Reverse Phase Arrays

Epigenomics

  • DNA methylation arrays
  • Chromatin immunoprecipitation (ChIP) arrays

Novel Applications

  • Tissue arrays
  • Quantum Dots
  • Carbohydrate Arrays
  • Chemical Compound Arrays

Image of Molecular dvices Gene Pix 4100A and Spectramax M5 microplate reader spectrophotometer

Spectramax M5 microplate reader Spectrophotometer

The optical systems use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to
that of dedicated single-mode readers.

Source: Molecular Devices: SpectraMax M Series Multi-Mode Microplate Readers Manual and Molecular Devices GenePix 4100A Microarray Scanner website

Perkin Elmer Tri Carb 3110TR Liquid Scintillation Analyzer

The Tri-Carb® 3110TR is a computer-controlled benchtop liquid scintillation analyzer for detecting small amounts of alpha, beta and gamma radioactivity.

 

Source: PerkinElmer Tri-Carb 3110TR Low Activity Liquid Scintillation Analyzer Spec Sheet

Image of the Tri Carb 3110TR liquid Scintillation Analyzer

 

Applied Biosystems Hitachi 3500 Genetic Analyzer

The Applied Biosystems 3500/3500xL Genetic Analyzers are fluorescence based DNA analysis instrument using capillary electrophoresis technology with 8- or 24-capillaries.

Source: Applied Biosystems 3500/3500XL Genetic Analyzer User Guide
Image of the Hitachi 3500 Applied Biosystems Genetic Analyzer

 

Beckman CEQ8000XL Automated DNA Sequencer

This system is fully automated and capable of determining the base sequence and fragment length of DNA samples that have been prepared with dye-labeled reagents. Four-color, dye-labeled terminator chemistry kits are used to process samples for base sequence analysis. Generation of samples for fragment length analysis is performed using dye-labeled primers.

Source: CEQTM 8000 Genetic Analysis System User's Guide

Image of Beckman CEQ8000XL Automated DNA Sequencer

 

The Biomek® 3000 Laboratory Automation Workstation

The Biomek® 3000 Laboratory Automation Workstation is a multi-axis liquid handling instrument designed for benchtop use and to fit in a laminar flow or fume hood for sterile or hazardous operations. The open architecture design, along with the extensible operating software, provides a foundation for integrating current and future specific-use components.

The Biomek 3000 workstation is a single head instrument with a series of interchangeable tools. Different tools provide options for performing a variety of functions, including liquid transfer and plate washing operations and moving labware around the deck. The modular design of the Biomek 3000 workstation allows expansion of the instrument capabilities to include additional operations such as filtration, plate shaking, photometric microplate measurement, and high-capacity operation.

Image of the Beckman Biomek 3000 Laboratory Automation Workstation

Image of the Beckman Biomek 3000 Laboratory Automation Workstation

A variety of labware and hardware adapt the deck of the Biomek 3000 workstation to accomplish multiple tasks, ranging from performing simple labware positioning and liquid transfers to completing complex assays that typically require additional devices in the laboratory.

Source: Beckman Coutler Biomek® 3000 Laboratory Automation Workstation User's Manual

 

Coy Anaerobic Chamber

Used for anerobic microbiology research, these units are also critical to many other research areas such as protein purification, clinical microbiology, biochemistry, cell culture, human microbiome studies, biofuels and more. 

Source: Coylab.com/products/anareobic-chambers
 Image of student in front of Coy Anaerobic Chamber

 

Leica Microsystems Microscope

Precise 5W LED illumination ideal for cell and tissue culture, micromanipulation, and live cell examinations.

Source: Leica Microsystems
Image of student looking through the Leica Microsystems Microscope 

Molecular Devices Gene Pix 4100A  

Genomics

Transcriptomics

Proteomics

Epigenomics

Novel Applications

Source: Moleculardevices.com

Spectramax M5 microplate reader Spectrophotometer

The optical systems use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to
that of dedicated single-mode readers.

Source: Spectramax M Series Multi-Mode Microplate Readers User Manual

Image of Molecular dvices Gene Pix 4100A and Spectramax M5 microplate reader spectrophotometer

Seal Analytical AA3 Dissolved Nutrient Analyzer

Based on air Segmented Flow Analysis techniques (also known as Continuous Flow Analysis), the SEAL AutoAnalyzer 3 fully automates repetitive and complex sample analysis steps, from start-up to shutdown. The SEAL AA3 HR chemistry analyzer can also perform solvent extraction, distillation, gas diffusion, on-line filtration and in-line UV digestion in a continuously flowing stream. SEAL AA3 HR chemistry analyzers are fully supported with a library of over 700 documented applications including USEPA, ISO and ASTM standard methods.

Source: SealAnalytical.com

Image of Seal Analytical AA3 dissolved nutrient analyer

Image of Seal Analytical AA3 dissolved nutrient analyzer

 

XROMM Analyzer

X-Ray Reconstruction of Moving Morphology (XROMM) is a 3D imaging technology for visualizing rapid skeletal movement in vivo

XROMM combines 3D models of bone morphology with movement data from biplanar x-ray video to create highly accurate (±0.1 mm) re-animations of the 3D bones moving in 3D space. 

Rapid bone motion, such as during bird flight, frog jumping, and human running, can be visualized and quantified with XROMM.

Source: Xromm.org

Image of the XROMM Analyzer

Image of the XROMM Analyzer mobile c-arm fluroscope

 

Agilent 1260 High Performance Liquid Chromatograph (HPLC) with diode array detector 

A pressure range of up to 600 bar with a flow rate up to 
5 mL/min allows the use of almost any column – conventional, sub-2 µm-particle or superficially porous columns. A UV, fluorescence or ELSD detector provides the data rate required for high resolution separations or fast analyses. 

Source: labwrench.com

Image of Agilent 1260 High Performance LIquid Chromatograph HPLC with diode array detector

Agilent 7890B Gas Chromatographs (GC) with autosamplers and a variety of detectors: ECD, TCD, FID, MSD

Gas chromatography, coupled with static
headspace sampling, is an easy-to-use,
high-throughput tool for determining
residual solvent impurities in pharmaceutical
products. Sample preparation is simple, and
the method is easily validated. In addition,
headspace sampling allows you to avoid
matrix injections that can cause column
degradation and coelution.

Source: Agilent 7890B Gas Chromatograph brochure

 Image of Agilent 7890B Gas Chromatographs (GC) with autosmaplers and a variety of detectors: ECD, TCD, FID, MSD

Image of Agilent 7890B Gas Chromatographs (GC) with autosamplers and a variety of detectors: ECD, TCD, FID, MSD

 

 

Agilent Cary Eclipse Fluorescence Spectorphotometer

The fibre optic capabilities extend the utility of the system for simplified measurement of solid samples outside the sample compartment or for the direct measurement of cold, hot, or potentially dangerous samples, down to very low volumes.

Food
  • Additives and supplements. Quality
    Assurance testing and monitoring
  • Authenticity and food origin
  • Food packaging and processing
    including hydrocarbon contamination
    through processing
Environmental
  • Preliminary Identification of hydrocarbon
    origin in oil spills using synchronous
    scanning
  • Waste water tracing into environmental
    flows by using fluorescent markers
  • Investigation of the source of organic
    matter pollution in river and sea water

Image of Agilent Cary Eclipse Flurescence Spectorphotometer

Chemical and Materials Applications
  • Determination of fluorescent properties
    of cleaning products
  • Investigation of the fluorescent
    properties of optical components
  • Analysis of surface contamination by
    fluorescent organic compounds in
    manufacturing processes
  • Polarised (anisotropy) measurements
    for understanding the molecular
    environment during polymer research
Source: Agilent Cary Eclipse Fluorescence Spectrophotometer spec sheet

Agilent 240 FS AA Flame Atomic Absorption Spectrophotometer Hollow Cathode Lamps

Atomic absorption spectrophotometry analyzes the concentration of elements in a liquid sample based on energy absorbed from certain wavelengths of light (usually 190 to 900 nm). Atomic absorption spectrophotometers typically include a flame burner to atomize the sample (most commonly a hollow cathode lamp), a monochromator, and a photon detector. Equipped with a turret or fixed lamp socket that can hold multiple lamps (up to eight) to reduce downtime between samples or allow for sequential analysis.

Typical sensitivity for an atomic absorption spectrometer using a flame burner is in the parts per million range. For trace analysis, a graphite furnace can be used in place of a flame burner to increase the sensitivity by several orders of magnitude (in the parts per billion range). Atomic absorption spectrophotometers are used in many industries including environmental testing, metal analysis, semiconductor manufacturing, petroleum and chemical production, and in pharmaceuticals, for example.

Source: labcompare.com

Image of Agilent 240 FS AA Flame Atomic Absorption Spectrophotometer Hollow Cathode Lamps

Bruker Advance 400 MHz Nuclear Magnetic Resonance (NMR) Spectrometer

Analytical nuclear magnetic resonance (NMR) solutions and instruments for life science and material research applications.

Nuclear magnetic resonance spectroscopy is used to study the structure of molecules, the interaction of various molecules, the kinetics or dynamics of molecules and the composition of mixtures of biological or synthetic solutions or composites.

The size of the molecules analyzed can range from a small organic molecule or metabolite, to a mid-sized peptide or a natural product, all the way up to proteins of several tens of kDa in molecular weight.

NMR nuclear spectroscopy complements other structural and analytical techniques such as X-ray, crystallography and mass spectrometry. NMR’s advantage is the unique ability of a nuclear spectrometer to allow both the non-destructive and the quantitative study of molecules in solution and in solid state, as well as to enable the study of biological fluids.

Source: bruker.com

 

 IMage of Bruker Advance 400 MHz NMR Spectrometer

CEM MARS 6 Microwave system

Microwave is a technique used to dissolve solid sample matrices into an aqueous liquid. This is achieved by placing a sample in a concentrated acid matrix in a closed vessel and exposing it to microwave irradiation. Both the speed of thermal decomposition of the sample, and the solubility of metals are increased. Once these metals are in solution, they can be quantified through spectroscopic techniques. The MARS 6 acid digestion process routinely cuts the time of sample preparation by 50 – 75 % as compared to hot plates and hot block.

Source: CEM.com

Image of the CEM MARS 6 Microwave System

 

Dionex 5000 Ion Chromatographs (IC)

Ideally suited for trace level determinations with high matrix concentrations, using a standard or microbore column in the first dimension to separate analytes from matrix, followed by a capillary separation in the second dimension. This method provides high sensititivy, enabling conductivity detection limits to rival those of mass speectrometry.

Source: ThermoFisher.com 

 

Image of Dionex 500 Ion Chromatograph (IC)

 

 

TA Instruments Q10 Differential Scanning Calorimeter (DSC)

The most common DSC application is the precise measurement of transition temperature.
Whether a melting temperature of a polymer
or the polymorphic transition of a pharmaceutical,
DSC provides the information quickly and easily on a minimum amount of sample. Important temperature measurements include:

  • Melting Temperature
  • Glass Transition Temperature
  • Thermal Stability Temperature
  • Oxidation Onset Temperature
  • Cure Onset Temperature
  • Crystallization Temperature
  • Polymorphic Transition Temperature
  • Liquid Crystal Temperature
  • Protein Denaturation Temperature
  • Solid-Solid Transition Temperature
Source: tainstruments.com/brochure

Image of Beckman CEQ8000XL Automated DNA Sequencer

Teledyne Leeman Labs Hydra II Mercury Analyzer

It is a fully automated turnkey analyzer that measures mercury in solid and semi-solid sample matrices directly without any acid digestion (sample preparation). The system employs the technique of sample combustion (thermal decomposition), mercury concentration via gold amalgamation and detection by cold vapor atomic absorption.

Source: teledyneleemanlabs.com

Image of Teledyne Leeman Labs Hydra II Mercury Analyzer

 

Thermo Nicolet iS5 FTIR with ATR attachment

Infrared spectroscopy is the analytical technique for rapid identification of unknown materials (solids, liquids and gases) and product quality screening. 

Image of Thermo Nicolet iS5 FTIR with ATR attachment

Source: fishersci.com

Image of Thermo Nicolet iS5 FTIR with ATR attachment

 

Thermo Nicolet iS5 FTIR Spectrometer with ATR and multi-range beam splitter

The Nicolet iS50 analyzes complex mixtures and materials using its GC-IR or TGA-IR capabilities. The new OMNIC Mercury software automatically isolates and identifies compounds eluted from a GC column or evolved from a TGA. This functionality is especially useful in TGA, where several compounds can evolve at the same time. Such analyses provide insight into subtle formulation differences in plastics and rubbers, which can lead to not-so-subtle differences in product performance and quality.

Source: News-medical.net

IMage of Thermo Nicolet iS50 FTIR with ATR and multi-range beam splitter

 

Rigaku MiniFlex 600 X-ray Diffractometer (powder) with 6-sample changer

MiniFlex X-ray diffractometer (XRD) is a multipurpose analytical instrument that can determine: phase identification and quantification, percent (%) crystallinity, crystallite size and strain, lattice parameter refinement, Rietveld refinement, and molecular structure. It is widely used in research, especially in material science and chemistry, as well as in industry for research and quality control.

Source: Rigaku.com 

Image of Rigaku MiniFlex 600 X-ray Diffractometer (powder) with 6-sample changer

Rigaku XtaLAB mini X-ray Diffractometer (single crystal) (XRD)

The Rigaku XtaLAB, benchtop X-ray crystallography system, is a compact single crystal X-ray diffractometer designed to produce publication-quality 3D structures. Rapidly analyze new compounds as they are synthesized in the lab.

Source: Rigaku.com

IMage of Rigaku XtaLAB mini X-Ray Diffractometer (single crystal)

Coming Soon

 

 

Coming Soon

 

 

Coming Soon

 

 

NanoMagnetics Atomic Force Microscope (AFM)

Create images of atoms and map the surface structure materials. Offers Excellent features such as alignment-free design, closed loop flexure scanner, decoupled z scanner, 10 MP video microscope and flexible operating modes.

Source: Nanomagnetics Atomic Force Microscope Flyer

 Image of Stockton University physics instrumentation, atomic force microscope

More Coming Soon

 
 

Coming Soon

 

 

Facilities

The biology research and teaching facilities include a nucleic acid hybridization facility, an aseptic cell and tissue culture facility, three walk-in environmentally controlled chambers. Biology instrumentation includes an automated capillary DNA sequencer, a quantitative real time PCR, and analysis facilities, RNA and DNA automated electrophoresis systems, protein electrophoresis systems, RNA and DNA lab on chip analysis, along with numerous centrifuges including multiple high-speed units. The electronic capabilities of these rooms encourage faculty to experiment with innovative teaching techniques. Support spaces also include biology and chemistry preparation laboratories, marine and fresh water aquaria, animal room facilities, a mechanic shop and a stock room.

The F-Wing facility is also home to a prized Lord & Burnham Greenhouse that holds award winning collections of tropical and temperate specimens used in teaching and research. Two different temperate zones are used to create artificial microenvironments for orchids, cacti, ferns, and other plant species. Students enrolled in biology and related general studies courses, and faculty conducting research help contribute to the magnificent plant collection. Awards include the Pennsylvania Horticulture Society Award of Merit, The American Orchid Society Trophy for Outstanding Exhibit, The American Orchid Society Gold Medal for the Stockton-Waldor Orchid exhibit at the 1993 Philadelphia Flower Show, The Ocean City Flower Show Best Orchid Awards in 1994 and 2002.

The Physics Program is located in lower C-Wing where two computer-equipped introductory laboratories, two advanced laboratories, a project room, a seminar room and a lecture demonstration room are located. Some of the newer laboratory equipment includes: complete Zeeman Effect apparatus, Excelsior 532nm, 50mW, diode pumped solid state laser with a TMC high performance, self leveling vibration isolation optical bench, working models of the human body, and a Gretch-Ken sound booth for acoustical experiments. Nearby, the College has an astronomical observatory with a 0.4 m Meade LX200 Schmidt-Cassegrain computer-controlled telescope.

The Arts and Sciences Building provides substantial space for the teaching of Environmental Studies, Geology and related courses. A state-of-the- art computer lab with 25 powerful workstations allows easy access to such advanced technologies as Geographic Information Systems (GIS) and Global Positioning Systems. The Earth Resources laboratory lets students study soil and rock structure, map terrain and investigate interactions between the physical and biological environments. Similarly, water resources facilities provide opportunities for laboratory work in such areas as groundwater flow and water pollution. The Biological Resources laboratory is complemented by a greenhouse that provides teaching and research space, an insectary for raising specimens, and a collection room to prepare and study the specimens. A grant from the National Science Foundation funded the installation of remote weather stations on the campus for the long-term collection of such data as temperature, humidity and sunlight. A second grant from the National Science Foundation supported curricular revisions incorporating use of appropriate technologies into the curriculum with the goal of using the computer facilities and field data collection technology in all labs. Another grant supported the expansion of the computer facilities for teaching smaller sections of courses that utilize GIS.

The outstanding laboratory facilities, student and faculty research areas, and offices are complemented by a lecture hall that accommodates about 80 students and offers the latest in electronic accessories. It is designed to allow faculty to project information from a variety of sources and to use computers for demonstrations. A smaller lecture room similarly serves the needs of smaller classes in a warm, welcoming atmosphere, and a large teaching room with tables provides space that can be adapted to laboratories or demonstrations. Approximately 400 acres of the 1,600-acre campus have been set aside for an arboretum, a forestry nursery, ecologic succession plots and a study preserve. The nearby fields, woodlands marshes and inland and coastal waters offer additional learning opportunities. Faculty members take advantage of the College’s geographical location to offer a variety of field experiences, both in core courses and in advanced instruction.

The Marine Science and Environmental Field Station, located just eight miles from campus in the Jacques Cousteau National Estuarine Research Reserve, is an integral part of the Natural Sciences and Mathematics facilities. The facility is located on eight acres and features a teaching and research laboratory, a physical sciences laboratory and an aquaculture/wet laboratory. A 2009 renovation of all buildings has greatly increased opportunities for faculty-led research programs and general instruction. The facility also houses the offices of the Field Station Manager and the Coastal Research Center. Waterfront dockage for the fleet of five research vessels (ranging from 16-28 feet) and a dockside storage building for sampling equipment are also on site.

Natural Sciences and Mathematics faculty utilize the facility to offer courses containing field work and laboratory components from the Marine Science, Environmental Studies, Biology and General Studies programs. Available equipment for field instruction includes various nets and trawls, advanced water monitoring instruments, benthic sampling devices, marine surveying equipment, side scan sonar, and considerable support items for all field disciplines. The Teaching and Research laboratories feature the necessary equipment for chemical, biological and physical sciences instruction. Eight computer workstations are available, each featuring ArcView GIS software. Computer technology has been carefully integrated into the mathematics and science curricula.

In addition to the campus computer network, MacIntosh and IBM compatible computers are used widely. Software for many applications (including data collection, computer-aided design [CAD], geographic information systems [GIS] analyses and problem solving) is readily available. In addition to the main science laboratories and satellite facilities, several specialized laboratories on the campus include an audiology laboratory, a speech pathology clinic, a management simulation laboratory and multipurpose laboratories and research facilities for physical therapy, occupational therapy and nursing. Students are responsible for knowing and following all laboratory regulations regarding proper procedures and safety. They are instructed in these regulations, which are posted in the various laboratories and are strictly enforced by the faculty and staff.